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makeTCR_Hs.TRBV29-1_v2
(Plasmid #256417)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 256417 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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Backbone

  • Vector backbone
    pmakeTCR
  • Backbone manufacturer
    custom
  • Backbone size w/o insert (bp) 1710
  • Total vector size (bp) 2037
  • Vector type
    Synthetic Biology

Growth in Bacteria

  • Bacterial Resistance(s)
    Chloramphenicol, 25 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    TRBV29-1
  • Species
    H. sapiens (human), Synthetic
  • Insert Size (bp)
    321
  • Entrez Gene
    TRBV29-1 (a.k.a. TCRBV29S1, TCRBV4S1A1T, TRBV291)

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Replacement for first version of Hs.TRBV29 -1 (Plasmid #234011), switching from TCTG to TGTA assembly site at the TRBV-CDR3b junction to simplify makeTCR system.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    makeTCR_Hs.TRBV29-1_v2 was a gift from Michael Platten (Addgene plasmid # 256417 ; http://n2t.net/addgene:256417 ; RRID:Addgene_256417)

  • For your References section:

    makeTCR: A Modular Platform for Rapid, Flexible, Scalable, Single-Step T Cell Receptor Synthesis. Hamberger M, Neuhoff M-T, Pietrantonio SV, Boschert T, Maldonado Torres C, Errerd A, Tan CL, Lindner JM, Platten M, Green EW. bioRxiv 2025.04.27.647198 10.1101/2025.04.27.647198
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