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CTp130-Reporter-NT
(Plasmid #256440)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 256440 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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Backbone

  • Vector backbone
    Unspecified
  • Vector type
    Mammalian Expression
  • Selectable markers
    GFP

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Trans-splicing module with half of GFP-2A-BFP with non-targeting spacer
  • gRNA/shRNA sequence
    TCACCAGAAGCGTACCATACTCACGAACAG
  • Species
    Synthetic
  • Promoter SFFV

Cloning Information

  • Cloning method Gibson Cloning

Resource Information

Terms and Licenses

Trademarks:

  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    CTp130-Reporter-NT was a gift from Patrick Hsu (Addgene plasmid # 256440 ; http://n2t.net/addgene:256440 ; RRID:Addgene_256440)

  • For your References section:

    Rewriting endogenous human transcripts with dual CRISPR-guided 3' trans-splicing. Chandrasekaran SS, Tau C, Fu BXH, Nemeth M, Bartie L, Pawluk A, Konermann S, Hsu PD. Cell Syst. 2026 Feb 18;17(2):101487. doi: 10.1016/j.cels.2025.101487. Epub 2026 Feb 6. 10.1016/j.cels.2025.101487 PubMed 41653914
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