Rewriting endogenous human transcripts with dual CRISPR-guided 3' trans-splicing.
Chandrasekaran SS, Tau C, Fu BXH, Nemeth M, Bartie L, Pawluk A, Konermann S, Hsu PD
Cell Syst. 2026 Feb 18;17(2):101487. doi: 10.1016/j.cels.2025.101487. Epub 2026 Feb 6.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 256438 | CTp041-dCasRx-mCherry | Mammalian expression of a human codon optimized catalytically inactive CasRx |
| 256439 | CTp092-Cas7/11-BFP | Mammalian expression of a human codon optimized Cas7/11 with cloning site for spacer under U6 promoter |
| 256440 | CTp130-Reporter-NT | Mammalian expression of a TSM with half of GFP-2A-BFP with non-targeting spacer |
| 256441 | CTp132-Reporter-T | Mammalian expression of a TSM with half of GFP-2A-BFP with best targeting spacer |
| 256442 | CTp152-TSM-Backbone | Mammalian expression of a TSM with XTEN-GFP cargo with cloning site for a spacer with RfxCas13d spacer |
| 256443 | pSC084-Cas7/11-mCherry | Mammalian expression of a human codon optimized Cas7/11 with cloning site for spacer under U6 promoter |
| 256444 | pSC135-TSM-Cargo-Backbone-GFP | Mammalian expression of a TSM with XTEN-GFP cargo with cloning sites for a cargo sequence and a spacer with RfxCas13d spacer |