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BdPMT::LUC
(Plasmid #256447)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 256447 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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Backbone

  • Vector backbone
    pIPKb001
  • Vector type
    Luciferase

Growth in Bacteria

  • Bacterial Resistance(s)
    Spectinomycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    EPI300
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Luciferase
  • Species
    Firefly
  • Promoter PMT

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site SwaI (not destroyed)
  • 3′ cloning site AscI (not destroyed)
  • 5′ sequencing primer GAGAGATTTAAATGATGCGGAGGAGATAG
  • 3′ sequencing primer GAGAGGCGCGCCTAAGCTTGCATGCTCTAGC
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please visit https://doi.org/10.1101/2025.04.03.647122 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    BdPMT::LUC was a gift from Samuel Hazen (Addgene plasmid # 256447 ; http://n2t.net/addgene:256447 ; RRID:Addgene_256447)

  • For your References section:

    Temperature signals drive grass secondary cell wall thickening. Gregory GA, Coomey JH, Khahani B, Gonze D, Omran S, McGillivray KA, Stewart CE, Gardner KA, Follette D, Hazen SP. bioRxiv [Preprint]. 2025 Apr 6:2025.04.03.647122. doi: 10.1101/2025.04.03.647122. 10.1101/2025.04.03.647122 PubMed 40236254
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