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pBac(EFA-Brainbow; 3xP3-DsRed)
(Plasmid #256611)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 256611 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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Backbone

  • Vector backbone
    pBac(3xP3-DsRed)
  • Backbone manufacturer
    Horn & Wimmer 2000, Dev Genes Evol 210: 623–629
  • Vector type
    piggyBac transposon in plasmid backbone

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert 1

  • Gene/Insert name
    Brainbow 1.1M (with mutated Kusabira) expressed by EFA cis-regulatory element
  • Species
    Synthetic
  • Mutation
    coding sequence of Kusabira orange fluorescent protein has been disrupted

Gene/Insert 2

  • Gene/Insert name
    3xP3-DsRed as transformation marker
  • Species
    Synthetic

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Brainbow 1.1 M comes from Livet et al. 2007 Nature 450: 56-62; EFA regulatory element comes from Sarrazin et al. 2012 Science 336: 338-41; pBac(3xP3-EGFP) comes from Horn & Wimmer 2000, Dev Genes Evol 210: 623–629.

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please visit https://doi.org/10.64898/2026.05.11.724235 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pBac(EFA-Brainbow; 3xP3-DsRed) was a gift from Michalis Averof (Addgene plasmid # 256611 ; http://n2t.net/addgene:256611 ; RRID:Addgene_256611)
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