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TREt-Kras(G12D)-PGK-rtTA3
(Plasmid #256624)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 256624 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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Backbone

  • Vector backbone
    SB pT2 B/H
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    30°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Unknown

Gene/Insert 1

  • Gene/Insert name
    Kras
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    567
  • Mutation
    G12D
  • Entrez Gene
    Kras (a.k.a. K-Ras, K-Ras 2, K-ras, Ki-ras, Kras-2, Kras2, c-K-ras, c-Ki-ras, p21B, ras)
  • Promoter Ef1a

Cloning Information for Gene/Insert 1

Gene/Insert 2

  • Gene/Insert name
    rTertR
  • Insert Size (bp)
    747
  • Promoter PGK

Cloning Information for Gene/Insert 2

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    TREt-Kras(G12D)-PGK-rtTA3 was a gift from Wen Xue (Addgene plasmid # 256624 ; http://n2t.net/addgene:256624 ; RRID:Addgene_256624)

  • For your References section:

    KRAS Withdrawal in Cholangiocarcinoma Leads to Immune Infiltration and Tumor Regression. Qiao Y, Yee MF, Parikh CN, Ma B, Cao Y, Shih YH, Wu JQ, Ruscetti M, Liang SQ, Xue W. Adv Sci (Weinh). 2026 Jan;13(6):e11312. doi: 10.1002/advs.202511312. Epub 2025 Dec 3. 10.1002/advs.202511312 PubMed 41332325
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