sgOPTI:U6-gRNA-blastR
(Plasmid
#256626)
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Purposelenti virus vector expressing a Cas9 guide RNA under a human U6 promoter. Contains blast resistance cassette.
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 256626 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
Backbone
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Vector backbonesgOpti
- Backbone size w/o insert (bp) 8950
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Vector typeLentiviral
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature30°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namesgRNA
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Alt namesingle guide RNA
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gRNA/shRNA sequenceN/A
- Promoter U6 promoter
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer N/A
- (Common Sequencing Primers)
Resource Information
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please visit https://doi.org/10.1101/2025.09.16.676677 for bioRxiv preprint.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
sgOPTI:U6-gRNA-blastR was a gift from Jesse Engreitz (Addgene plasmid # 256626 ; http://n2t.net/addgene:256626 ; RRID:Addgene_256626)
Map uploaded by the depositor.
