pcDNA5/FRT/TO-N-mEGFP-NUDT12
(Plasmid
#256706)
-
PurposeFor transient expression of NUDT12 in mammalian cells or for generation of stable lines using FlpIn compatible cells
-
Depositing Lab
-
Depositing OrganizationUniversitaetsklinikum Aachen
Located in Germany -
Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 256706 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
Backbone
-
Vector backbonepcDNA5
-
Backbone manufacturerInvitrogen
-
Vector typeMammalian Expression
Growth in Bacteria
-
Bacterial Resistance(s)Ampicillin, 100 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)DH5alpha
-
Copy numberHigh Copy
Gene/Insert
-
Gene/Insert nameNUDT12
-
Alt nameNUDIX12
-
SpeciesH. sapiens (human)
-
Insert Size (bp)1386
-
Mutationfl with stop codon
-
Entrez GeneNUDT12 (a.k.a. DKFZp761I172)
-
Tag
/ Fusion Protein
- mEGFP (N terminal on insert)
Cloning Information
- Cloning method Gateway Cloning
- 5′ sequencing primer EGFPC
- (Common Sequencing Primers)
Resource Information
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
pcDNA5/FRT/TO-N-mEGFP-NUDT12 was a gift from Karla Feijs (Addgene plasmid # 256706 ; http://n2t.net/addgene:256706 ; RRID:Addgene_256706)