pDEST17-NUDT6
(Plasmid
#256709)
-
PurposeFor bacterial expression and purification of NUDT6 with a His tag
-
Depositing Lab
-
Depositing OrganizationUniversitaetsklinikum Aachen
Located in Germany -
Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 256709 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
Backbone
-
Vector backbonepDEST17
-
Backbone manufacturerInvitrogen
-
Vector typeBacterial Expression
Growth in Bacteria
-
Bacterial Resistance(s)Ampicillin, 100 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)DH5alpha
-
Copy numberHigh Copy
Gene/Insert
-
Gene/Insert nameNUDT6
-
Alt nameNUDIX6
-
SpeciesH. sapiens (human)
-
Insert Size (bp)948
-
Mutation48-311 with stop codon
-
Entrez GeneNUDT6 (a.k.a. ASFGF2, FGF-AS, FGF2AS, GFG-1, GFG1)
-
Tag
/ Fusion Protein
- 6xHIS (N terminal on backbone)
Cloning Information
- Cloning method Gateway Cloning
- 5′ sequencing primer T7
- (Common Sequencing Primers)
Resource Information
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
pDEST17-NUDT6 was a gift from Karla Feijs (Addgene plasmid # 256709 ; http://n2t.net/addgene:256709 ; RRID:Addgene_256709)