pRB2461
(Plasmid #256749)

• Purpose: (Empty Backbone) C-terminal fusions of C. albicans optimized smURFP fluorescent protein.
• Depositing Lab: Richard Bennett
• Publication: Ke et al Sci Rep (2026)

Backbone

• Vector backbone: pSFS2A
• Vector type: Synthetic Biology ; Candida albicans expression
• Selectable markers: Nourseothricin
• Tag / Fusion Protein:
  • smURFP (C terminal on insert)

Growth in Bacteria

• Bacterial Resistance(s): Chloramphenicol, 25 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: None
• Mutation: Codon-optimized for Candida albicans
• Tag / Fusion Protein:
  • smURFP (C terminal on insert)

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Please note that the depositor's annotated reference file has minor differences compared to the Addgene verified sequence. The different orientation of the F1 ori does not alter the plasmid's function. Please refer to the the Addgene sequence for the most accurate result.

Please visit https://doi.org/10.64898/2026.05.11.723596 for bioRxiv preprint.