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pZE21-blaPYX-2
(Plasmid #256753)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 256753 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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Backbone

  • Vector backbone
    pZE21-MCS
  • Backbone manufacturer
    EXPRESSYS
  • Backbone size w/o insert (bp) 2254
  • Total vector size (bp) 2959
  • Modifications to backbone
    KpnI site mutated to BsiWI
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    pZE21-blaPYX-2
  • Species
    Pyxidicoccus sp. 3LG
  • Insert Size (bp)
    765
  • Mutation
    none
  • GenBank ID
    PQ855797.1
  • Promoter pTetO/L
  • Tag / Fusion Protein
    • none (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BsiWI (not destroyed)
  • 3′ cloning site BamHI (not destroyed)
  • 5′ sequencing primer TGGCAATTCCGACGTCTAAG
  • 3′ sequencing primer GTCTAGGGCGGCGGATTTG
  • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pZE21-blaPYX-2 was a gift from Stathis Kotsakis (Addgene plasmid # 256753 ; http://n2t.net/addgene:256753 ; RRID:Addgene_256753)

  • For your References section:

    Predatory myxobacteria lay at the cross-roads of metallo-beta-lactamase evolution. Lambropoulou A, Pavlidi A, Samiotaki M, Tammam MA, Ioannou E, Roussis V, Tzouvelekis LS, Miriagou V, Kotsakis SD. Commun Biol. 2026 Jun 12. doi: 10.1038/s42003-026-10466-8. 10.1038/s42003-026-10466-8 PubMed 42286160
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