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pET26b(+)-blarecMYX-1
(Plasmid #256755)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 256755 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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Backbone

  • Vector backbone
    pET26b(+)
  • Backbone manufacturer
    Novagen
  • Backbone size w/o insert (bp) 5300
  • Total vector size (bp) 6002
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    blaMYX-1
  • Species
    Myxococcus xanthus
  • Insert Size (bp)
    702
  • Mutation
    Deletion of nucleotides 1-80 and silent mutation C429A
  • GenBank ID
    NG_057453.1
  • Promoter T7 promoter
  • Tag / Fusion Protein
    • PelB signal peptide (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NcoI (destroyed during cloning)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer GGCTGCACATCACGGTGG
  • 3′ sequencing primer AGTAGCGCGCCCTGCCGT
  • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET26b(+)-blarecMYX-1 was a gift from Stathis Kotsakis (Addgene plasmid # 256755 ; http://n2t.net/addgene:256755 ; RRID:Addgene_256755)

  • For your References section:

    Predatory myxobacteria lay at the cross-roads of metallo-beta-lactamase evolution. Lambropoulou A, Pavlidi A, Samiotaki M, Tammam MA, Ioannou E, Roussis V, Tzouvelekis LS, Miriagou V, Kotsakis SD. Commun Biol. 2026 Jun 12. doi: 10.1038/s42003-026-10466-8. 10.1038/s42003-026-10466-8 PubMed 42286160
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