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pJS668
(Plasmid #257116)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 257116 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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Backbone

  • Vector backbone
    pRSF-Duet
  • Backbone size w/o insert (bp) 3700
  • Total vector size (bp) 7859
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert 1

  • Gene/Insert name
    lptD P. aeruginosa
  • Species
    Pseudomonas aeruginosa PAO1
  • Insert Size (bp)
    2775
  • GenBank ID
    PA0595
  • Promoter T7

Cloning Information for Gene/Insert 1

Gene/Insert 2

  • Gene/Insert name
    lptE
  • Species
    Pseudomonas aeruginosa PAO1
  • Insert Size (bp)
    645
  • Mutation
    Codon-optimized for E. coli expression
  • GenBank ID
    PA3988
  • Tag / Fusion Protein
    • 8x His-tag (C terminal on insert)

Gene/Insert 3

  • Gene/Insert name
    lptM
  • Species
    E. coli
  • Insert Size (bp)
    228
  • GenBank ID
    JW3781
  • Tag / Fusion Protein
    • Flag-tag (C terminal on insert)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please visit https://doi.org/10.21203/rs.3.rs-8115403/v1 for Research Square preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pJS668 was a gift from Sebastian Hiller (Addgene plasmid # 257116 ; http://n2t.net/addgene:257116 ; RRID:Addgene_257116)

  • For your References section:

    Structural basis for antibiotics murepavadin and thanatin targeting the lipopolysaccharide insertase. Lehner PA, Rožić L, Modaresi SM, Pluhackova K, Zbinden P, Obrecht D, Sedzicki J, Hiller S. Research Square [Preprint] 2025. doi: https://doi.org/10.21203/rs.3.rs-8115403/v1 10.21203/rs.3.rs-8115403/v1
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