pCIG4
(Plasmid #257131)

• Purpose: (Empty Backbone) Expression vector with pCAGGS promoter and IRES-EGFP, Kan backbone
• Depositing Lab: Anthony-Samuel LaMantia
• Depositing Organization: Virginia Polytechnic Institute and State University
  Located in the United States of America
• Publication: pCAGGS derived expresssion vectors used in primary culture experiments (unpublished)

Backbone

• Vector backbone: pCAG/pCIG
• Backbone size (bp): 5200
• Modifications to backbone: Derived from pCAGGS/pCIG vectors; composite promoter (CMV + Chicken beta actin + rabbit beta-globin splice acceptor) was joined with the IRES-EGFP fragment from pIRES2-EGFP (Clonetech). This was placed nto a KAN backbone spliced from pGBKT7. Additional modifications added an C-terminal HA tag reading frame, substituted the EGFP-N1 multicloning site, and removed several stray restriction sites for easier fragment cloning.
• Vector type: Mammalian Expression
• Promoter: pCAGG (composite CMV + Chicken beta-actin)
• Tags / Fusion Proteins:
  • HA (C terminal on insert)
  • IRES-EGFP (C terminal on backbone)

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Cloning Information

• Cloning method: Restriction Enzyme

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry