Skip to main content
Addgene
Open Menu

pAAV-Ef1a-fDIO-tTA-P2A-TurboRFP-WPRE
(Plasmid #257137)

Print

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 257137 Standard format: Plasmid sent in bacteria as agar stab 1 $94
Add to Cart

Backbone

  • Vector backbone
    pAAV
  • Total vector size (bp) 7105
  • Vector type
    Mammalian Expression, AAV

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    30°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    tTA-P2A-TurboRFP
  • Species
    Synthetic
  • Insert Size (bp)
    1512
  • Promoter EF1a

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NheI (not destroyed)
  • 3′ cloning site AscI (not destroyed)
  • 5′ sequencing primer WPRE_R
  • 3′ sequencing primer EF1a_F
  • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    The tTA-P2A-TurboRFP sequence was cloned from pK168.AAV-EF1a-DIO-tTA-P2A-RFP-WPRE (Addgene #85039) and the backbone was derived from pAAV-EF1a-fDIO-mCherry (Addgene #114471).

Terms and Licenses

Trademarks:

  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pAAV-Ef1a-fDIO-tTA-P2A-TurboRFP-WPRE was a gift from Kazutaka Ikeda (Addgene plasmid # 257137 ; http://n2t.net/addgene:257137 ; RRID:Addgene_257137)

  • For your References section:

    AAV-Based Bright and Sparse Labeling of Versatile Neurons Adaptable in Cre-Dependent Genetic Backgrounds. Kamijo S, Ikeda K, Miwa H. eNeuro. 2026 Mar 27;13(3):ENEURO.0314-25.2026. doi: 10.1523/ENEURO.0314-25.2026. Print 2026 Mar. 10.1523/ENEURO.0314-25.2026 PubMed 41839576
Related items: