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pAAV-TRE-Flpe-WPRE
(Plasmid #257138)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 257138 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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Backbone

  • Vector backbone
    pAAV
  • Total vector size (bp) 5725
  • Vector type
    Mammalian Expression, AAV

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    30°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Flpe
  • Species
    S. cerevisiae (budding yeast)
  • Insert Size (bp)
    1294

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site SalI (not destroyed)
  • 3′ cloning site HindIII (not destroyed)
  • 5′ sequencing primer Flpe (backward)
  • 3′ sequencing primer WPRE_R
  • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    The Flpe sequence was cloned from pK036.TRE-Flpe-WPRE (Addgene #85007), and the backbone was derived from pK170.AAV-TRE-Cre-WPRE (Addgene #85040).

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pAAV-TRE-Flpe-WPRE was a gift from Kazutaka Ikeda (Addgene plasmid # 257138 ; http://n2t.net/addgene:257138 ; RRID:Addgene_257138)

  • For your References section:

    AAV-Based Bright and Sparse Labeling of Versatile Neurons Adaptable in Cre-Dependent Genetic Backgrounds. Kamijo S, Ikeda K, Miwa H. eNeuro. 2026 Mar 27;13(3):ENEURO.0314-25.2026. doi: 10.1523/ENEURO.0314-25.2026. Print 2026 Mar. 10.1523/ENEURO.0314-25.2026 PubMed 41839576
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