E5BBQ3_b.45_rd_h90_ba_10_19_ICCG
(Plasmid
#257153)
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PurposeExpression construct for E5BBQ3_b.45_rd_h90_ba_10_19_ICCG gene, codon optimized for expression in E. coli
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 257153 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
Backbone
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Vector backbonepCDB179
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameE5BBQ3_b.45_rd_h90_ba_10_19_ICCG
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SpeciesOther
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MutationS19R, N28S, R46T, E47T, N48T, N49G, Y51F, E64Q, A65S, E72P, I87N, Q92G, E98R, N101L, N105D, H106Y, M107L, I108T, S121A, R138E, L139A, D145S, T165R, D174E, L175N, K186E, I196L, S197D, D204C, T207S, F209I, I213S, K216T, I217T, G219A, V223I, R245S, E253C
Cloning Information
- Cloning method Gene Synthesis
Resource Information
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
E5BBQ3_b.45_rd_h90_ba_10_19_ICCG was a gift from Gregg Beckham (Addgene plasmid # 257153 ; http://n2t.net/addgene:257153 ; RRID:Addgene_257153)