TfCut2_b.45_h90_t.33_i2200_12_19
(Plasmid
#257156)
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PurposeExpression construct for TfCut2_b.45_h90_t.33_i2200_12_19 gene, codon optimized for expression in E. coli
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 257156 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
Backbone
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Vector backbonepCDB179
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameTfCut2_b.45_h90_t.33_i2200_12_19
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SpeciesOther
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MutationR18S, S19R, E27T, R46T, E47T, N49G, Y51F, G62A, E64Q, A65S, I74L, I82F, E98T, N101L, N105D, H106Y, M107C, I108T, N109E, S121A, L139A, S141E, Q142A, D145S, L175G, T183Q, I196L, S197E, T207S, I213S, K216T, I217T, T237E, R245K
Cloning Information
- Cloning method Gene Synthesis
Resource Information
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
TfCut2_b.45_h90_t.33_i2200_12_19 was a gift from Gregg Beckham (Addgene plasmid # 257156 ; http://n2t.net/addgene:257156 ; RRID:Addgene_257156)