OlexA_mini35S_AvrPtoB_SYFP2_pENTR_D-TOPO
(Plasmid
#257233)
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PurposeA cloning backbone designed for the insertion of GOI (genes of interest) to be C-terminal SYFP2 tagged
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 257233 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
Backbone
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Vector backbonepENTR/DTOPO
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Vector typeGateway Cloning
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameOlexA_mini35S_AvrPtoB_SYFP2
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SpeciesPseudomonas syringae (AvrPtoB)
- Promoter OlexA_mini35S
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Tag
/ Fusion Protein
- SYFP2 (C terminal on insert)
Cloning Information
- Cloning method Other
- 5′ sequencing primer M13/pUC Forward
- 3′ sequencing primer M13/pUC Reverse
- (Common Sequencing Primers)
Resource Information
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
OlexA_mini35S_AvrPtoB_SYFP2_pENTR_D-TOPO was a gift from On Sun Lau (Addgene plasmid # 257233 ; http://n2t.net/addgene:257233 ; RRID:Addgene_257233) -
For your References section:
Stomatal XVE: an inducible system for cell-stage-specific gene expression and editing in the stomatal lineage. Kou S, Chua LC, Tan JQ, Lau OS. New Phytol. 2026 Apr;250(2):1330-1347. doi: 10.1111/nph.71017. Epub 2026 Feb 15. 10.1111/nph.71017 PubMed 41693245