pCAG-TSF-GFP-WIPI3
(Plasmid
#257242)
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PurposeExpressing GFP-taged human full length WIPI3
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Depositing Lab
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Depositing OrganizationUniversity of California, Berkeley
Located in the United States of America -
Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 257242 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
Backbone
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Vector backbonepCAG
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameWDR45B
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Alt nameNEDSBAS
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Alt nameWIPI-3
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SpeciesH. sapiens (human)
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Entrez GeneWDR45B (a.k.a. NEDSBAS, WDR45L, WIPI-3, WIPI3)
- Promoter CAG
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Tag
/ Fusion Protein
- TSF-GFP (N terminal on insert)
Cloning Information
- Cloning method Gene Synthesis
- 5′ sequencing primer GTTCGGCTTCTGGCGTGT
- (Common Sequencing Primers)
Resource Information
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCAG-TSF-GFP-WIPI3 was a gift from James Hurley (Addgene plasmid # 257242 ; http://n2t.net/addgene:257242 ; RRID:Addgene_257242) -
For your References section:
Reconstitution of multistep recruitment of ULK1 to membranes in autophagy. Duan Y, Lu Y, Paul S, Betz J, Wilhelm L, Cook ASI, Ren X, Adriaenssens E, Martens S, Ganley IG, Hummer G, Hurley JH. bioRxiv [Preprint]. 2025 Nov 9:2025.11.07.687251. doi: 10.1101/2025.11.07.687251. 10.1101/2025.11.07.687251 PubMed 41278708