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PCDH1-HA-ATG101-WF-DD
(Plasmid #257244)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 257244 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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Backbone

  • Vector backbone
    pCDH1-CMV-HT-LC3-SV40-Hygro
  • Vector type
    Mammalian Expression, Lentiviral
  • Selectable markers
    Hygromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    ATG101
  • Alt name
    C12orf44
  • Species
    H. sapiens (human)
  • Mutation
    Trp110, Phe112 to Asp
  • GenBank ID
    NP_068753.2
  • Entrez Gene
    ATG101 (a.k.a. C12orf44)
  • Tag / Fusion Protein
    • HA (N terminal on insert)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    PCDH1-HA-ATG101-WF-DD was a gift from James Hurley (Addgene plasmid # 257244 ; http://n2t.net/addgene:257244 ; RRID:Addgene_257244)

  • For your References section:

    Reconstitution of multistep recruitment of ULK1 to membranes in autophagy. Duan Y, Lu Y, Paul S, Betz J, Wilhelm L, Cook ASI, Ren X, Adriaenssens E, Martens S, Ganley IG, Hummer G, Hurley JH. bioRxiv [Preprint]. 2025 Nov 9:2025.11.07.687251. doi: 10.1101/2025.11.07.687251. 10.1101/2025.11.07.687251 PubMed 41278708
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