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FINICI-SykAR-kras_WT
(Plasmid #257345)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 257345 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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Backbone

  • Vector backbone
    pCDNA3'
  • Backbone size w/o insert (bp) 5500
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    FINICI SykAR wildtype, non-raft targeted
  • Alt name
    FINICI-SykAR-kras_WT
  • Species
    Synthetic
  • Insert Size (bp)
    1926
  • Promoter CMV
  • Tags / Fusion Proteins
    • 6xHis (N terminal on insert)
    • CAAX (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site unknown (unknown if destroyed)
  • 5′ sequencing primer CMV_F
  • 3′ sequencing primer BGH_R
  • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    FINICI-SykAR-kras_WT was a gift from Gary Mo (Addgene plasmid # 257345 ; http://n2t.net/addgene:257345 ; RRID:Addgene_257345)
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