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pET gamma-syt7C2AB-mRuby
(Plasmid #257617)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 257617 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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Backbone

  • Vector backbone
    pET28a(+)
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    Depositors recommend using E. coli strain BL21 (DE3).
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    syt7 and mRuby3
  • Species
    M. musculus (mouse)
  • Mutation
    N/A
  • Entrez Gene
    Syt7 (a.k.a. B230112P13Rik)

Cloning Information

  • Cloning method Other

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Constructed with In-Fusion cloning.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET gamma-syt7C2AB-mRuby was a gift from Edwin Chapman (Addgene plasmid # 257617 ; http://n2t.net/addgene:257617 ; RRID:Addgene_257617)

  • For your References section:

    Alternative splicing of synaptotagmin 7 regulates oligomerization and short-term synaptic plasticity. Mehta N, Larson DT, Salaka RJ, Wozney M, Subramani S, Mishra S, Kaur S, Jain A, Chapman ER. bioRxiv [Preprint]. 2025 Oct 28:2025.10.27.684894. doi: 10.1101/2025.10.27.684894. 10.1101/2025.10.27.684894 PubMed 41278746
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