NL813
(Plasmid
#257627)
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PurposePuromycin resistance and H2B-mCherry expression for Salpingoeca rosetta
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 257627 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
Backbone
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Vector backboneNK802
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Vector typeS. rosetta expression
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Selectable markersPuromycin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameH2B-linker
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SpeciesSynthetic
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Insert Size (bp)390
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Tag
/ Fusion Protein
- mCherry
Resource Information
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please visit https://doi.org/10.64898/2026.04.05.716596 for bioRxiv preprint.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
NL813 was a gift from Nicole King (Addgene plasmid # 257627 ; http://n2t.net/addgene:257627 ; RRID:Addgene_257627) -
For your References section:
Septins regulate cytokinesis and multicellular development in the closest living relatives of animals. Carver M, King N. bioRxiv [Preprint]. 2026 Apr 7:2026.04.05.716596. doi: 10.64898/2026.04.05.716596. 10.64898/2026.04.05.716596 PubMed 41993322