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pCDFDuet-1:MCS1_NedB_MCS2_NedC
(Plasmid #257772)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 257772 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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Backbone

  • Vector backbone
    pCDFDuet-1
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Streptomycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    NedB and NedC
  • Species
    Neisseria dentiae CCUG 53898 310003
  • Promoter T7

Cloning Information

  • Cloning method Gibson Cloning

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please visit https://doi.org/10.1101/2025.10.31.685832 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCDFDuet-1:MCS1_NedB_MCS2_NedC was a gift from Wilfred van der Donk (Addgene plasmid # 257772 ; http://n2t.net/addgene:257772 ; RRID:Addgene_257772)

  • For your References section:

    Co-opting the bacterial lipoprotein pathway for the biosynthesis of lipidated macrocyclic peptides. Chen JY, Zhu L, Zhang KY, Berthold DA, van der Donk WA. Proc Natl Acad Sci U S A. 2026 Apr 7;123(14):e2532672123. doi: 10.1073/pnas.2532672123. Epub 2026 Mar 30. 10.1073/pnas.2532672123 PubMed 41911463
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