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pET28a:His-BsfD
(Plasmid #257774)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 257774 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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Backbone

  • Vector backbone
    pET28a
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    BsfD
  • Species
    Burkholderia mayonis sp. BDU6
  • Promoter T7
  • Tag / Fusion Protein
    • 6xHis (N terminal on backbone)

Cloning Information

  • Cloning method Gibson Cloning

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please visit https://doi.org/10.64898/2026.01.15.699783 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET28a:His-BsfD was a gift from Wilfred van der Donk (Addgene plasmid # 257774 ; http://n2t.net/addgene:257774 ; RRID:Addgene_257774)

  • For your References section:

    Discovery of the Phosphonate Flavophos Produced by Burkholderia. Simon MA, Ramos-Figueroa JS, Reyes Lopez V, Ongpipattanakul C, Zhu L, Giurgiu C, Hoffpauir ZA, Lamb AL, Nair SK, van der Donk WA. J Am Chem Soc. 2026 May 6;148(17):18030-18043. doi: 10.1021/jacs.6c01748. Epub 2026 Apr 26. 10.1021/jacs.6c01748 PubMed 42036871
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