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pRSFDuet-1:MCS1_His-Amm12_Amm13
(Plasmid #257776)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 257776 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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Backbone

  • Vector backbone
    pRSFDuet-1
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Amm12 and Amm13
  • Species
    Streptomyces sp CNR698
  • Promoter T7
  • Tag / Fusion Protein
    • 6xHis (N terminal on backbone)

Cloning Information

  • Cloning method Gibson Cloning

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please visit https://doi.org/10.1101/2025.09.03.674030 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pRSFDuet-1:MCS1_His-Amm12_Amm13 was a gift from Wilfred van der Donk (Addgene plasmid # 257776 ; http://n2t.net/addgene:257776 ; RRID:Addgene_257776)

  • For your References section:

    The Biosynthetic Pathway to the Pyrroloiminoquinone Marine Natural Product Ammosamide C. Ramos-Figueroa JS, Zhu L, Halliman M, van der Donk WA. J Am Chem Soc. 2025 Dec 17;147(50):46315-46323. doi: 10.1021/jacs.5c15504. Epub 2025 Dec 5. 10.1021/jacs.5c15504 PubMed 41348511
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