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pETDuet-PbsA4
(Plasmid #257781)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 257781 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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Backbone

  • Vector backbone
    pETDuet
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    PbsA4
  • Species
    Peribacillus simplex BE23
  • Promoter T7
  • Tag / Fusion Protein
    • 6xHis (N terminal on backbone)

Cloning Information

  • Cloning method Gibson Cloning

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pETDuet-PbsA4 was a gift from Wilfred van der Donk (Addgene plasmid # 257781 ; http://n2t.net/addgene:257781 ; RRID:Addgene_257781)

  • For your References section:

    Biosynthesis of Biphenomycin-like Macrocyclic Peptides by Formation and Cross-Linking of Ortho-Tyrosines. Padhi C, Zhu L, Chen JY, Huang C, Moreira R, Challis GL, Cryle MJ, van der Donk WA. J Am Chem Soc. 2025 Jul 9;147(27):23781-23796. doi: 10.1021/jacs.5c06044. Epub 2025 Jun 26. 10.1021/jacs.5c06044 PubMed 40568902
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