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pCS11
(Plasmid #257828)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 257828 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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Backbone

  • Vector backbone
    pR6K
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Spectinomycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Pir1
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    J23108::mRFP

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Elmore, Joshua R., et al. "High-throughput genetic engineering of nonmodel and undomesticated bacteria via iterative site-specific genome integration." Science Advances 9.10 (2023): eade1285.

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Alternative name: pAR038

Usage/Comments: This plasmid is a poly-attP payload integration vector. It contains multiple orthogonal attP sites compatible with cSAGE landing pads, allowing integration via different serine recombinases. The plasmid backbone is flanked by ΦC31 att sites. This plasmid contains a spectinomycin resistance cassette for organisms where kanamycin is ineffective. Please visit https://doi.org/10.64898/2026.04.17.717921 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCS11 was a gift from James Carothers (Addgene plasmid # 257828 ; http://n2t.net/addgene:257828 ; RRID:Addgene_257828)
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