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PssaB-sfGFP-mRuby2
(Plasmid #257838)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 257838 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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Backbone

  • Vector backbone
    pTU2-a-pSC101-Kan
  • Backbone manufacturer
    modified from EcoFlex library
  • Backbone size w/o insert (bp) 3751
  • Total vector size (bp) 5769
  • Vector type
    Bacterial Expression ; Golden Gate (EcoFlex)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 20 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert 1

  • Gene/Insert name
    PssaB-BBa_B0034-sfGFP-L3S2P21
  • Species
    Salmonella enterica serovar Typhimurium
  • Insert Size (bp)
    1106
  • Promoter PssaB

Cloning Information for Gene/Insert 1

Gene/Insert 2

  • Gene/Insert name
    SJM901-PET3a_RBS-mRuby2-Bba_B0015
  • Species
    Salmonella enterica serovar Typhimurium
  • Insert Size (bp)
    912
  • Promoter SJM901

Cloning Information for Gene/Insert 2

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    PssaB-sfGFP-mRuby2 was a gift from Keara Lane (Addgene plasmid # 257838 ; http://n2t.net/addgene:257838 ; RRID:Addgene_257838)

  • For your References section:

    Temporal tuning of switch-like virulence expression resolves environmental uncertainty through phenotypic heterogeneity. Spratt M., Lane K.. Current Biology, Volume 36, Issue 13, 2026, Pages 3336-3353.e8 ISSN 0960-9822 10.1016/j.cub.2026.06.001
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