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12x His-SUMO BRD4 (333-460) L387A + E438C "BromoCatch" from Homo Sapiens
(Plasmid #257846)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 257846 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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Backbone

  • Vector backbone
    pRSF-DUET1
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Bromodomain-containing protein 4
  • Species
    M. musculus (mouse)
  • Mutation
    Deleted amino acids 333-459 , Leucine 387 to Alanine, & Glutamic acid 483 to Cystine
  • GenBank ID
    P25440
  • Entrez Gene
    Brd4 (a.k.a. Brd5, HUNK1, MCAP, WI-11513)
  • Tag / Fusion Protein
    • 12x His SUMO (N terminal on insert)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    12x His-SUMO BRD4 (333-460) L387A + E438C "BromoCatch" from Homo Sapiens was a gift from Alessio Ciulli (Addgene plasmid # 257846 ; http://n2t.net/addgene:257846 ; RRID:Addgene_257846)

  • For your References section:

    BromoCatch: a self-labelling tag platform for protein modification and live cell imaging. Rodriguez-Rios M, Craigon C, Nakasone MA, Sathe G, Bond AG, Dorward M, Edmonds AK, Norley MC, Arnold RE, Wood PM, Reynolds SJ, Cresser-Brown JO, Marsh GP, Maple HJ, Ciulli A. Nat Commun. 2026 May 13. doi: 10.1038/s41467-026-72539-w. 10.1038/s41467-026-72539-w PubMed 42128888
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