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Upstream U6 24-mer acceptor backbone
(Plasmid #257852)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 257852 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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Backbone

  • Vector backbone
    PiggyBac_24mer_pegArray_bb
  • Backbone manufacturer
    Weissman Lab, Koblan et al. 2025 (Addgene plasmid #238545)
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    30°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    None

Resource Information

Terms and Licenses

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please visit https://doi.org/10.64898/2026.05.07.722278 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Upstream U6 24-mer acceptor backbone was a gift from Jonathan Weissman (Addgene plasmid # 257852 ; http://n2t.net/addgene:257852 ; RRID:Addgene_257852)

  • For your References section:

    Comprehensive lineage tracing maps the landscape of cell fate decisions in mouse embryogenesis. Colgan WN, Koblan LW, Villagrana J, Hou TJ, Wang M, Gowri G, Chandler W, Sepulveda LA, Ciftci D, Smolyar K, Yost KE, Young A, Wittler L, Markoulaki S, Loh KM, Zhuang X, Yosef N, Smith ZD, Weissman JS. Cell. 2026 Oct 8:S0092-8674(26)01196-7. doi: 10.1016/j.cell.2026.09.050. 10.1016/j.cell.2026.09.050 PubMed 42849436
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