pAAV-hSyn-DIO-dLight3.6
(Plasmid
#257856)
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PurposeCre-dependent expression of the green genetically encoded dopamine sensor dLight3.6 under synapsin promoter.
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 257856 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
Backbone
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Vector backbonepAAV
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Vector typeMammalian Expression, AAV, Cre/Lox
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature30°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namedLight3.6
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SpeciesSynthetic
- Promoter Synapsin
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site Unknown (unknown if destroyed)
Resource Information
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pAAV-hSyn-DIO-dLight3.6 was a gift from Lin Tian (Addgene plasmid # 257856 ; http://n2t.net/addgene:257856 ; RRID:Addgene_257856) -
For your References section:
Sensitive dLight3 for imaging broad-spectrum dopamine events across brain regions. Roshgadol JI, Chouinard JA, Majumder S, Scott EC, Borges K, Hagihara KM, Mancini N, Steveson T, Kamath T, Lodder B, MacLennan BJ, Dalangin R, Tjahjono N, Pal A, Soares-Cunha C, Melugin PR, Marley A, Mahe K, Kurima K, Takahashi S, Nosaka D, Murakami K, Colgan LA, Freitas PT, Chaudhuri R, Siciliano CA, Rodrigues AJ, Gradinaru V, Von Zastrow M, Podgorski K, Sabatini BL, Bidaye SS, Hanks TD, Ji N, Wickens JR, Inagaki HK, Tian L. Res Sq [Preprint]. 2025 Aug 20:rs.3.rs-7313638. doi: 10.21203/rs.3.rs-7313638/v1. 10.21203/rs.3.rs-7313638/v1 PubMed 40894030