pTolDEST_TRE: iCre_int_R4R3 (AJ38)
(Plasmid
#257881)
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PurposeTol2 Gateway DEST plasmid with a TRE: iCre-dA cassette to make conditional transgenics
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Depositing Lab
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Publication
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 257881 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
Backbone
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Vector backbonepTolDEST_R4R3 (Gateway)
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Backbone manufacturerInvitrogen
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Vector typeCre/Lox, Gateway Cloning ; vertebrate expression
Growth in Bacteria
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Bacterial Resistance(s)Chloramphenicol and Ampicillin, 25 & 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)ccdB Survival
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameTRE: iCre expression cassette
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SpeciesSynthetic
- Promoter TRE
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site BamHI (destroyed during cloning)
- 3′ cloning site BamHI (destroyed during cloning)
Resource Information
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A portion of this plasmid was derived from a plasmid made byInvitrogen
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pTolDEST_TRE: iCre_int_R4R3 (AJ38) was a gift from Abigail Jensen (Addgene plasmid # 257881 ; http://n2t.net/addgene:257881 ; RRID:Addgene_257881)