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pTolDEST_TRE: iCre_int_R4R3 (AJ38)
(Plasmid #257881)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 257881 Standard format: Plasmid sent in bacteria as agar stab 1 $94

Backbone

  • Vector backbone
    pTolDEST_R4R3 (Gateway)
  • Backbone manufacturer
    Invitrogen
  • Vector type
    Cre/Lox, Gateway Cloning ; vertebrate expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Chloramphenicol and Ampicillin, 25 & 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    ccdB Survival
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    TRE: iCre expression cassette
  • Species
    Synthetic
  • Promoter TRE

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (destroyed during cloning)
  • 3′ cloning site BamHI (destroyed during cloning)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Invitrogen

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pTolDEST_TRE: iCre_int_R4R3 (AJ38) was a gift from Abigail Jensen (Addgene plasmid # 257881 ; http://n2t.net/addgene:257881 ; RRID:Addgene_257881)