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pLVX-eGFP-STING-mut-RRAA
(Plasmid #257888)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 257888 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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Backbone

  • Vector backbone
    pLVX
  • Total vector size (bp) 8329
  • Vector type
    Lentiviral

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    eGFP_ms_STING_mut_RRAA
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    1137
  • Mutation
    changed Arginine 330 and 333 to Alanine
  • Promoter eF1a
  • Tag / Fusion Protein
    • eGFP (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoR1 (not destroyed)
  • 3′ cloning site Mlu1 (not destroyed)
  • 5′ sequencing primer GGTTCATTCTCAAGCCTCAG
  • 3′ sequencing primer CGAGGTCG ACGGTATCGA TG
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLVX-eGFP-STING-mut-RRAA was a gift from Michel Desjardins (Addgene plasmid # 257888 ; http://n2t.net/addgene:257888 ; RRID:Addgene_257888)
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