Sc Pol31-pET(16b) (no tag)
(Plasmid
#257902)
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PurposeOver-express Sc Pol31 in E. coli. To be used in conjunction with Sc GST-P-Pol32-pLANT(2b)/RIL (Addgene #257903) to over-express Sc [Pol31 + GST/P-Pol32] complex in E. coli.
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Depositing Lab
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Publication
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 257902 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
Backbone
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Vector backbonepET(16b)
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberLow Copy
Gene/Insert
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Gene/Insert nameSc Pol31
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SpeciesS. cerevisiae (budding yeast)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site Unspecified (unknown if destroyed)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
Sc Pol31-pET(16b) (no tag) was a gift from Michael O'Donnell (Addgene plasmid # 257902 ; http://n2t.net/addgene:257902 ; RRID:Addgene_257902)
