Sc GST-P-Pol32-pLANT(2b)/RIL
(Plasmid
#257903)
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PurposeOver-express Sc GST/P-Pol32 in E. coli. To be used in conjunction with Sc Pol31-pET(16b) (no tag) (Addgene #257902) to over-express Sc [Pol31 + GST/P-Pol32] complex in E. coli.
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Depositing Lab
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Publication
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 257903 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
Backbone
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Vector backbonepLANT(2b)
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberLow Copy
Gene/Insert
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Gene/Insert nameSc Pol32
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SpeciesS. cerevisiae (budding yeast)
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Tag
/ Fusion Protein
- GST tag followed by PreScission Protease site (N terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site Unspecified (unknown if destroyed)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
Sc GST-P-Pol32-pLANT(2b)/RIL was a gift from Michael O'Donnell (Addgene plasmid # 257903 ; http://n2t.net/addgene:257903 ; RRID:Addgene_257903)