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UtPEv1-SlOR (PE8533)
(Plasmid #257965)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 257965 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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Backbone

  • Vector backbone
    pLSL.R.Ly
  • Backbone manufacturer
    Jae-Yean Kim Lab
  • Backbone size w/o insert (bp) 6791
  • Total vector size (bp) 19417
  • Vector type
    Plant Expression, CRISPR, Synthetic Biology
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    EPI300
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    UtPEv1
  • gRNA/shRNA sequence
    pegR-SlOR
  • Species
    Synthetic
  • Promoter CaMV35S

Cloning Information

  • Cloning method Golden Gate
  • 5′ sequencing primer ggcgcgtgccgaattcggat
  • 3′ sequencing primer cccactgacttgaagtacac
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    UtPEv1-SlOR (PE8533) was a gift from Jae-Yean Kim (Addgene plasmid # 257965 ; http://n2t.net/addgene:257965 ; RRID:Addgene_257965)

  • For your References section:

    Development of an ultra-efficient prime editing system in tomato. Van Vu T, Thi Nguyen N, Kim J, Hoai Nguyen T, Kim JY. Nat Commun. 2026 Jan 12;17(1):95. doi: 10.1038/s41467-025-67874-3. 10.1038/s41467-025-67874-3 PubMed 41526382
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