pLenti-CRISPRv2-sgENPP1_#1-hygro
(Plasmid
#258011)
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PurposeENPP1 sgRNA with Cas9 and hygromycin resistance
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Depositing Lab
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Publication
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 258011 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
Backbone
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Vector backbonepLenti-CRISPRv2
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Vector typeLentiviral, CRISPR
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Selectable markersHygromycin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namesgRNA with Cas9 and hygromycin resistance
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gRNA/shRNA sequenceATATGACTGTACCCTACGGG
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SpeciesM. musculus (mouse)
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Entrez GeneEnpp1 (a.k.a. 4833416E15Rik, CD203c, E-NPP 1, E-NPP1, Ly-41, M6S1, NPP1, Npps, PC-1, Pca, Pca-1, Pdnp1, ttw, twy)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pLenti-CRISPRv2-sgENPP1_#1-hygro was a gift from Brian Ruffell (Addgene plasmid # 258011 ; http://n2t.net/addgene:258011 ; RRID:Addgene_258011)