pHL0130
(Plasmid
#258142)
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PurposeExpresses E. coli csgA, the major subunit of curli fibers, under IPTG induction.
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 258142 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
Backbone
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Vector backbonepTACxc
- Total vector size (bp) 6844
Growth in Bacteria
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Bacterial Resistance(s)Chloramphenicol, 25 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pHL0130 was a gift from Aaron Whiteley (Addgene plasmid # 258142 ; http://n2t.net/addgene:258142 ; RRID:Addgene_258142) -
For your References section:
Functional amyloid proteins confer defence against predatory bacteria. Ledvina HE, Sayegh R, Carale RO, Burroughs AM, Macklin AR, Azadeh AL, Borja Najera LD, Aravind L, Whiteley AT. Nature. 2025 Aug;644(8075):197-204. doi: 10.1038/s41586-025-09204-7. Epub 2025 Jul 2. 10.1038/s41586-025-09204-7 PubMed 40604283