pCAG-Raygun-eGFP.03
(Plasmid
#258158)
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PurposeExpression of miniaturized eGFP variant in mammalian cells
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Depositing Lab
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Publication
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 258158 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
Backbone
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Vector backbonepCAG
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameeGFP-templated Raygun variant
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SpeciesSynthetic
- Promoter CAG
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Tag
/ Fusion Protein
- NLS (N terminal on insert)
Cloning Information
- Cloning method Other
Resource Information
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCAG-Raygun-eGFP.03 was a gift from Scott Soderling (Addgene plasmid # 258158 ; http://n2t.net/addgene:258158 ; RRID:Addgene_258158) -
For your References section:
Miniaturizing, Modifying, and Magnifying Nature’s Proteins with Raygun. Devkota K, Shonai D, Mao J, Ko YS, Wang W, Soderling S, Singh R. bioRxiv 2024.08.13.607858 10.1101/2024.08.13.607858