mCherry-FTL
(Plasmid
#258164)
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PurposeExpresses FTL N terminally tagged with mCherry
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 258164 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
Backbone
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Vector backbonepBABEpuro-gateway
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Backbone manufacturerMatthew Meyerson (Addgene # 51070)
- Backbone size w/o insert (bp) 7611
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Vector typeRetroviral
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Selectable markersPuromycin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameFTL
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Alt nameNBIA3
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Alt nameFerritin Light Polypeptide-Like 3
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SpeciesH. sapiens (human)
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Insert Size (bp)630
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Entrez GeneFTL (a.k.a. FTL1, LFTD, NBIA3)
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Tag
/ Fusion Protein
- mCherry (N terminal on insert)
Cloning Information
- Cloning method Gateway Cloning
- 5′ sequencing primer NA
- (Common Sequencing Primers)
Resource Information
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A portion of this plasmid was derived from a plasmid made bypBABE puro-mCherrry-GTW, was engineered by cloning mCherry2 (Addgene # 54563) into pBABEpuro-gateway (Addgene # 51070)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
mCherry-FTL was a gift from Heidi McBride (Addgene plasmid # 258164 ; http://n2t.net/addgene:258164 ; RRID:Addgene_258164)