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pRUTH5_single-chainH2B-H2A
(Plasmid #258232)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 258232 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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Backbone

  • Vector backbone
    pRUTH5
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    H2B-H2A
  • Alt name
    Histone H2B-H2A
  • Species
    X. laevis (frog)
  • Tag / Fusion Protein
    • 6xHis-TEV (N terminal on insert)

Cloning Information

  • Cloning method Ligation Independent Cloning

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pRUTH5_single-chainH2B-H2A was a gift from David Shechter (Addgene plasmid # 258232 ; http://n2t.net/addgene:258232 ; RRID:Addgene_258232)

  • For your References section:

    Structure of a single-chain H2A/H2B dimer. Warren C, Bonanno JB, Almo SC, Shechter D. Acta Crystallogr F Struct Biol Commun. 2020 May 1;76(Pt 5):194-198. doi: 10.1107/S2053230X20004604. Epub 2020 Apr 28. 10.1107/S2053230X20004604 PubMed 32356520
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