pLV[Exp]-EF1A>3xFLAG/3xGGGGS /mMboat2[NM_026037.3]
(Plasmid
#258331)
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PurposeExpresses mouse Mboat2 [NM_026037.3] with 3XFLAG in a lentiviral expression vector
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Depositing Lab
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Publication
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 258331 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
Backbone
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Vector backbonepLV
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Backbone manufacturerVectorBuilder
- Total vector size (bp) 9042
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Vector typeMammalian Expression, Lentiviral
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberLow Copy
Gene/Insert
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Gene/Insert nameMboat2
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SpeciesM. musculus (mouse)
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GenBank IDNM_026037.3
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Entrez GeneMboat2 (a.k.a. 2810049G06Rik, LPCAT4, Moact2, Oact2)
- Promoter EF1α
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Tag
/ Fusion Protein
- FLAG (N terminal on insert)
Cloning Information
- Cloning method Gene Synthesis
- 5′ sequencing primer Ef1a_F
- (Common Sequencing Primers)
Resource Information
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A portion of this plasmid was derived from a plasmid made bySynthesized by VectorBuilder
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pLV[Exp]-EF1A>3xFLAG/3xGGGGS /mMboat2[NM_026037.3] was a gift from Xiaoai Zhao (Addgene plasmid # 258331 ; http://n2t.net/addgene:258331 ; RRID:Addgene_258331)