pAAV-BglII
(Plasmid
#258367)
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PurposeCloning plasmid for HDR donor in rAAV-mediated gene editing
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Depositing Lab
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Publication
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 258367 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
Backbone
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Vector backbonepAAV-BglII
- Backbone size w/o insert (bp) 3496
- Total vector size (bp) 4993
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Vector typeAAV
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namepAAB-BglII
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Insert Size (bp)1497
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site BglII (not destroyed)
- 3′ cloning site BglII (not destroyed)
- 5′ sequencing primer M13F
- (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pAAV-BglII was a gift from Samuele Marro (Addgene plasmid # 258367 ; http://n2t.net/addgene:258367 ; RRID:Addgene_258367) -
For your References section:
CRISPR-Cas9 RNP and rAAV-Mediated Gene Editing in Human Pluripotent Stem Cells. Baljinnyam E, Grisanti L, Tattari S, Marro S. protocols.io 2026 10.17504/protocols.io.ewov1rpnylr2/v1