MGCO-27 tol2-IsceI-eef1al1:mStayGold:sec61b-SV40-attB-IsceI-tol2
(Plasmid
#258401)
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PurposemStayGold reporter for sec61b in zebrafish
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 258401 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
Backbone
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Vector backbonetol2-IsceI-eef1a1l1 promoter-attB-IsceI-tol2
- Backbone size w/o insert (bp) 4889
- Total vector size (bp) 8569
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Vector typezebrafish expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namesec61b
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SpeciesH. sapiens (human)
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Insert Size (bp)285
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Entrez GeneSEC61B
- Promoter eef1a1l1
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Tag
/ Fusion Protein
- mStayGold (N terminal on insert)
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer gcgagtcagtgagcgagg
- (Common Sequencing Primers)
Resource Information
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
MGCO-27 tol2-IsceI-eef1al1:mStayGold:sec61b-SV40-attB-IsceI-tol2 was a gift from Gokul Upadhyayula (Addgene plasmid # 258401 ; http://n2t.net/addgene:258401 ; RRID:Addgene_258401)
Map uploaded by the depositor.
