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plentiCRISPRv2 Notch1
(Plasmid #258451)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 258451 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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Backbone

  • Vector backbone
    plentiCRISPRv2
  • Backbone manufacturer
    Feng Zhang (Addgene #52961)
  • Vector type
    Mammalian Expression, Lentiviral
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    30°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Notch1 sgRNA
  • gRNA/shRNA sequence
    CGT_CAG_CGT_GAG_CAG_GTC_GC
  • Species
    Synthetic
  • Promoter U6

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Unspecified (unknown if destroyed)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    plentiCRISPRv2 Notch1 was a gift from Matthew Kutys (Addgene plasmid # 258451 ; http://n2t.net/addgene:258451 ; RRID:Addgene_258451)

  • For your References section:

    Notch1 cortical signaling regulates epithelial architecture and cell-cell adhesion. White MJ, Jacobs KA, Singh T, Mayo LN, Lin A, Chen CS, Jun YW, Kutys ML. J Cell Biol. 2023 Dec 4;222(12):e202303013. doi: 10.1083/jcb.202303013. Epub 2023 Oct 5. 10.1083/jcb.202303013 PubMed 37796194
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