pRRL SNAP-NOTCH1 TMD
(Plasmid
#258454)
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PurposeLentiviral expression of SNAP-tagged Notch 1 transmembrane domain
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 258454 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
Backbone
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Vector backbonepRRL
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Vector typeMammalian Expression, Lentiviral
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature30°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameNotch1 TMD
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SpeciesH. sapiens (human)
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Entrez GeneNOTCH1 (a.k.a. AOS5, AOVD1, TAN1, hN1)
- Promoter CMV
Cloning Information
- Cloning method Gibson Cloning
Resource Information
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pRRL SNAP-NOTCH1 TMD was a gift from Matthew Kutys (Addgene plasmid # 258454 ; http://n2t.net/addgene:258454 ; RRID:Addgene_258454) -
For your References section:
Notch1 cortical signaling regulates epithelial architecture and cell-cell adhesion. White MJ, Jacobs KA, Singh T, Mayo LN, Lin A, Chen CS, Jun YW, Kutys ML. J Cell Biol. 2023 Dec 4;222(12):e202303013. doi: 10.1083/jcb.202303013. Epub 2023 Oct 5. 10.1083/jcb.202303013 PubMed 37796194