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plentiCRISPRv2 DLL4
(Plasmid #258457)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 258457 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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Backbone

  • Vector backbone
    plentiCRISPRv2
  • Backbone manufacturer
    Feng Zhang (Addgene #52961)
  • Vector type
    Mammalian Expression, Lentiviral
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    30°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    DLL4 sgRNA
  • gRNA/shRNA sequence
    CAT_CAA_CGA_GCG_CGG_CGT_AC
  • Species
    Synthetic
  • Promoter U6

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Unspecified (unknown if destroyed)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    plentiCRISPRv2 DLL4 was a gift from Matthew Kutys (Addgene plasmid # 258457 ; http://n2t.net/addgene:258457 ; RRID:Addgene_258457)

  • For your References section:

    The Notch1 intracellular domain orchestrates mechanotransduction of fluid shear stress. Singh T, Jacobs KA, Polacheck WJ, Kutys ML. Life Sci Alliance. 2026 Feb 5;9(4):e202503599. doi: 10.26508/lsa.202503599. Print 2026 Apr. 10.26508/lsa.202503599 PubMed 41644301
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