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pRRL DLL4-GFP-P2A-HygroR
(Plasmid #258459)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 258459 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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Backbone

  • Vector backbone
    pRRL
  • Vector type
    Mammalian Expression, Lentiviral
  • Selectable markers
    Hygromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    30°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    DLL4
  • Species
    H. sapiens (human)
  • Entrez Gene
    DLL4 (a.k.a. AOS6, delta4, hdelta2)
  • Promoter CMV

Cloning Information

  • Cloning method Gibson Cloning

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please note that Addgene's sequencing results finds R478L in DLL4 (NP_061947.1). The depositor confirms that this change is not known to affect plasmid function.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pRRL DLL4-GFP-P2A-HygroR was a gift from Matthew Kutys (Addgene plasmid # 258459 ; http://n2t.net/addgene:258459 ; RRID:Addgene_258459)

  • For your References section:

    The Notch1 intracellular domain orchestrates mechanotransduction of fluid shear stress. Singh T, Jacobs KA, Polacheck WJ, Kutys ML. Life Sci Alliance. 2026 Feb 5;9(4):e202503599. doi: 10.26508/lsa.202503599. Print 2026 Apr. 10.26508/lsa.202503599 PubMed 41644301
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